Sample Type:Serum, plasma, tissue homogenates and other biological fluidsIntended Use:Galectin 14 (GAL14) BioAssay ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of GAL14 in human serum, plasma, tissue homogenates and other biological fluids.Sensitivity:<0.129ng/mlRange:0.312-20ng/mlSpecificity:Recognizes human GAL14. No significant cross-reactivity or interference between GAL14 and analogues was observed.Test Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to GAL14. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GAL14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GAL14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GAL14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Precision:Intra-Assay: CV<10%Inter-Assay: CV<12%Kit Components:Pre-coated, ready to use plate, 1x 96 wellsStandard, 2x vialsDetection Reagent A, 1×120ulDetection Reagent B, 1×120ulTMB Substrate, 1×9mlWash Buffer, 30X, 1×20mlPlate sealer for 96 wells, 4x sealerStandard Diluent, 1×20mlAssay Diluent A, 1×12mlAssay Diluent B, 1×12mlStop Solution, 1×6mlInstruction manual, 1x manualStorage and Stability:Desiccation recommended. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt. Store all other reagents at 4°C. Stable for 6 months after receipt of order.